Hydrogen peroxide accelerates senescence of human dental pulp stem cells

doi:10.3969/j.issn.2095-4344.2016.10.016

Abstract

Abstract:

BACKGROUND: The process of oxidative stress that impacts the curative effect exists in the region which accepts cell transplantation. However, there are few reports about the effects of oxidative stress on human dental pulp stem cells and relevant mechanism.

OBJECTIVE: To understand the effect of hydrogen peroxide on the senescence of human dental pulp stem cells.

METHODS: Human dental pulp stem cells were isolated and cultured in PBS, 100 and 200 μmol/L hydrogen peroxide for 2 hours, respectively. Cell morphology was observed under inverted microscope, degree of cell senescence monitored by β-galactosidase staining, cell proliferation ability detected by BrdU kit and cell counting method, cytoskeleton of dental pulp stem cells and expression of sirt1 tested using immunofluorescence method, and expression of sirt1 and p16 proteins measured by western blot assay.

RESULTS AND CONCLUSION: Dental pulp stem cells exhibited a fibroblast-like morphology with spindle-shaped appearance. After stimulated by hydrogen peroxide, the cell volume was enlarged, the β-galactosidase staining deepened and the proliferation of dental pulp stem cells reduced. The enhancement of senescence of dental pulp stem cells was accompanied with the increasing concentration of hydrogen peroxide, and in this process, the expression of p16 was raised while the expression of sirt1 was decreased. In conclusion, the senescence of human dental pulp stem cells can be promoted by the stimulation of hydrogen peroxide, and sirt1 and p16 are involved in this process. Our findings may provide a theoretical and experimental foundation for autologous transplantation of dental pulp stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Xu Ke, Feng Gui-juan, Feng Xing-mei, Huang Dan, Zheng Ke, Tang En-yi. Hydrogen peroxide accelerates senescence of human dental pulp stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1481-1487.

Figures/Tables 5

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